RTF VI: Sequence-based Profiling

Coordinators: P. Rosenstiel (CAU), A. Franke (CAU)

RTF VI "Sequence-based profiling" develops tools for molecular profiling of patients for precision medicine, and enables reliable access to sequencing methods and bioinformatic resources. Among other things, the structure is used for biomarker analysis in large cohorts of the cluster.

What does this research area's work build on?

RTF VI is based on extensive and long-term work (since 2007) on "Next Generation Sequencing" (NGS). The NGS platform in Kiel has been continuously expanded and enlarged in recent years, and is by now one of the largest academic facilities of its kind that is also one of the four central DFG-funded NGS Centers in Germany (Competence Center for Genome Analysis Kiel, CCGA). NGS data from the platform has been successfully used in numerous previous international and high-profile research projects, such as the 1000 Genomes Project, IHEC/DEEP, GEUVADIS, EASI-Genomics, READNA and the ICGC.

 

What are the main research objectives?

The research area provides flexible and scalable sequencing technologies in the fields of genetics, epigenetics, functional genomics and microbiome research. In particular, the required laboratory procedures are standardized, diagnostic tools and sequencing methods are developed and integrative approaches are established for genomics, proteomics, and metabolomics. Innovative approaches to examine complex tissues at the individual and single cell level are currently being tested.

 

What makes this research area special?

The size and experience of the platform, as well as its high national and international profile make RTF VI unique in the North of Germany. The range of laboratory protocols offered, i.e. the diversity of the portfolio, is another characteristic feature of the platform. The high-throughput capacity enables the processing of several thousand samples within a short period of time. The individual cell technologies facilitate the description of new cell types and states with the highest possible resolution.

 

What does the research area contribute to precision medicine in chronic inflammation?

RTF VI will provide high-resolution profiles for individual patients. This makes PMI patients "transparent" at the molecular level, and thereby able to be analyzed objectively. The researchers want to use their modern technologies not only to determine the causes of disease in individual patients, but also to find new biomarkers for the early detection of diseases and for treatment management. In addition, the members are tackling the question of how to clearly report and represent the complex data for doctors (diagnostic findings), and possibly also for those affected.

 

Cooperation with other research areas in the cluster

RTF I for genomics, RTF II for microbial analysis, RTF III for iPSC/organoid research and RTF V for the integration of proteomics and metabolomics. The samples for RTF VI primarily come from the clinical projects CD-1, CD-2 and CD-5.

 

Members

Prof. Dr. rer. nat. Norbert Arnold

Full member

UKSH Campus Kiel
Department of Gynecology and Obstetrics
Laboratory of Oncology

Prof. Dr. rer. nat. Petra Bacher

Full member

UKSH Campus Kiel
Department of Immunology

Prof. Dr. John Baines

Full member

Max Planck Institute for Evolutionary Biology
Institute of Experimental Medicine
Evolutionary Genomics

Prof. Dr. Lars Bertram

Full member

University of Lübeck
Lübeck Interdisciplinary Platform for Genome Analytics (LIGA)

Prof. Dr. David Ellinghaus

Full member

Kiel University
Institute of Clinical Molecular Biology
Centre for Molecular Biosciences

Prof. Dr. Jeanette Erdmann

Full member

University of Lübeck
Institute for Cardiogenetics

Dr. rer. nat. Maren Falk-Paulsen

Associated member

Kiel University
Institute of Clinical Molecular Biology
RG Prof. Rosenstiel

Prof. Dr. rer. nat. Andre Franke

Full member

Kiel University
Institute of Clinical Molecular Biology
Genetics & Bioinformatics

Prof. Dr. rer. nat. Robert Häsler

Full member

Kiel University
Institute of Clinical Molecular Biology
Molecular Cell Biology

Prof. Dr. Saleh Ibrahim

Full member

UKSH Campus Lübeck
Lübeck Institute for Experimental Dermatology
Genetics of Inflammatory Diseases

Prof. Dr. med. Ralf Junker

Full member

UKSH Campus Kiel
Institute of Clinical Chemistry
Central laboratory

PD Dr. Kathrin Kalies

Full member

University of Lübeck
Institute for Anatomy

Prof. Dr. med. Wolfram Klapper

Full member

UKSH Campus Kiel
Institute of Pathology
Department of Hematopathology

Prof. Dr. Inke König

Full member

University of Lübeck
Institute for Medical Biometrics and Statistics

Prof. Dr. rer. nat. Ben Krause-Kyora

Full member

Kiel University
Institute of Clinical Molecular Biology
Ancient DNA Research

Prof. Dr. rer. nat. Michael Krawczak

Full member

UKSH Campus Kiel
Institute of Medical Informatics and Statistics

Prof. Dr. Tanja Lange

Full member

UKSH Campus Lübeck
Department of Rheumatology and Clinical Immunology

Dr. rer. nat. Lennart T. N. Lenk

Associated member

UKSH Campus Kiel
Department of Child and Youth Medicine I

Dr. Tobias Lenz

Full member

Max Planck Institute for Evolutionary Biology
Evolutionary Immunogenomics

Dr. rer. nat. Britt-Sabina Löscher

Associated member

Kiel University
Institute of Clinical Molecular Biology
Centre for Molecular Biosciences

Prof. Dr. Thomas F. Meyer

Full member

UKSH Campus Kiel
Institute of Clinical Molecular Biology
Senior RG Infection Oncology

Priv.-Doz. Dr. Franz-Josef Müller

Full member

UKSH Campus Kiel
Department of Psychiatry and Psychotherapy
Center for Integrative Psychiatry gGmbH

Prof. Almut Nebel

Full member

Kiel University
Institute of Clinical Molecular Biology
Longevity Ancient DNA Research

Prof. Dr. Stefan Niemann

Full member

Research Center Borstel - Leibniz Lung Center
Molecular and Experimental Mycobacteriology
Priority Research Area Infections

Prof. Dr. Dirk Nowotka

Full member

Kiel University
Department of Computer Science
Dependable Systems

Prof. Dr. rer. nat. Alexander Scheffold

Full member

UKSH Campus Kiel
Department of Immunology

Prof. Dr. med. Denis Schewe

Full member

UKSH Campus Kiel
Department of Child and Youth Medicine I

Prof. Dr. med. Stefan Schreiber

Spokesperson

Kiel University
Institute of Clinical Molecular Biology

Prof. Dr. med. Dominik Schulte

Full member

UKSH Campus Kiel
Department of Internal Medicine I

Prof. Dr. Markus Schwaninger

Full member

University of Lübeck
Institute for Experimental and Clinical Pharmacology and Toxicology

Florian Tran

Associated member

UKSH Campus Kiel
Department of Internal Medicine I

Dr. Artem Vorobyev

Associated member

UKSH Campus Lübeck
Lübeck Institute for Experimental Dermatology
Model Systems of Inflammatory Skin Diseases

Prof. Dr. Xinhua Yu

Full member

Research Center Borstel - Leibniz Lung Center
Pulmonary Immune Diseases
Priority Research Area Asthma and Allergy